Journal
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
Volume 31, Issue 11, Pages 3259-3264Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.5012/bkcs.2010.31.11.3259
Keywords
Myeloperoxidase; Ionic liquid; Carbon nanotubes; Cerium dioxide; Immunosensor
Categories
Funding
- Science and Technology Development of Chongqing [CSTC-2009AC5145]
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A label-free amperometric immunosensor has been proposed for the detection of myeloperoxidase (MPO) in human serum To fabricate such an immunosensor, a composite film consisting of N,N-dimethylformamide (DMF), multi-wall carbon nanotubes (MWCNTs) and 1-ethyl-3-methyl imidazolium tetrafluoroborate (EMIMBF4) suspension was initially formed on a glassy carbon electrode (GCE) Then cerium dioxide (CeO2) dispersed by chitosan was coated on the GCE After that, MPO antibodies (anti-MPO) were attached onto the nanoCeO(2) surface With a noncompetitive immunoassay format, the antibody-antigen complex formed between the immobilized anti-M PO and MPO in sample solution The immunosensor was characterized by cyclic voltammetry, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) The factors influencing the performance of the immunosensor were studied in detail Under optimal conditions, the current change before and after the immunoreaction was proportional to MPO concentration in the range of 5 to 300 ng mL(-1) with a detection limit of 0 2 ng mL(-1)
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