Journal
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
Volume 31, Issue 8, Pages 2201-2206Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.5012/bkcs.2010.31.8.2201
Keywords
Cation-exchange; Metal chelate; Multidentate ligand; Binding capacity; Leakage
Categories
Funding
- Natural Science Foundation of Shaanxi Province [2007B22]
- Shaanxi Provincial Education Department [09JK758]
- Shannxi Provincial Key Discipline Program
- Scientific Research Foundation for the Master Program of Northwest University [09YSY24]
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To improve the separation property and stability of metal chelate Cu(II) column, three new kinds of multidentate amino-carboxy silica columns with cation-exchange properties were synthesized using glutamic acid (Glu), glutamic acid-bromoacetic acid (Glu-BAA), glutamic acid-bromosuccinic acid (Glu-BSUA) as ligands and silica gel as matrix. The standard proteins were separated with prepared chromatographic columns. The stationary phases exhibited the metal chelate property after fixing copper ion (II) on the synthesized multidentate ligand silica columns. The binding capacity of immobilized metal ion was related with the dentate number of multidentate ligands. Chromatographic behavior of proteins and the leakage of immobilized metal ion on multidentate chelate Cu(II) columns were affected by the dentate number of multidentate ligands and competitive elution system directly. The results showed that quinquedentate Glu-BSUA-Cu(II) column exhibited better chromatographic property and stability as compared with tridentate Glu-Cu(II) column, tetradentate Glu-BAA-Cu(II) column and commonly used IDA-Cu(II) column.
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