Journal
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 152, Issue 2, Pages 219-223Publisher
SPRINGER
DOI: 10.1007/s10517-011-1493-7
Keywords
L-asparaginase; antitumor enzyme; Yersinia pseudotuberculosis
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Recombinant E. coli strain producing Y. pseudotuberculosis Q66CJ2 (YpA) L-asparaginase II was created. Gene ansB homologue encoding Y. pseudotuberculosis IP 32953 L-asparaginase precursor was synthesized. The gene was cloned in pBad24 expression vector and expressed in E. coli BL21 (DE3) strain. Optimal conditions for the producer strain culturing were selected. An effective method for isolation and purification of the enzyme by two-staged column chromatography was developed.
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