Journal
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 150, Issue 2, Pages 180-184Publisher
SPRINGER
DOI: 10.1007/s10517-010-1099-5
Keywords
DNA aptamers; oligonucleotide thiophosphoryl analogs; anticoagulation effects; MALDI mass spectrometry
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Funding
- Russian Foundation for Basic Research [09-04-12133-ofi_m, 09-04-12249-ofi_m]
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In order to create effective therapeutically significant oligonucleotide structures, a series of analogs of thrombin-binding aptamer d(GGTTGGTGTGGTTGG) containing thiophosphoryl substitutions were synthesized. The anticoagulation effects of the resultant thrombin-binding aptamer analogs were evaluated and the effects of local thiomodifications on their structure and function were studied, including the effects on stability in blood plasma and resistance to DNA nucleases. A promising modified oligonucleotide (LL11) was found with the structure modified only in TT loops. It retains antithrombin properties of thrombin-binding aptamer, but is more resistant to biodegradation.
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