Journal
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 148, Issue 1, Pages 109-112Publisher
SPRINGER
DOI: 10.1007/s10517-009-0647-3
Keywords
stem cells; aneuploidy; clone formation; interphase nuclei; fluorescent in situ hybridization (FISH)
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Funding
- Russian Foundation for Basic Research [06-04-49135a, 07-04-12157-ofi]
- President of the Russian Federation [NSh-1006.2006.7]
- State Contract [02.512.11.2135]
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Cytogenetic analysis of 13 mesenchymal stem cell cultures isolated from normal human adipose tissue was carried out at different stages of culturing. The incidence of chromosomes 6, 8, 11, and X aneuploidy and polyploidy was studied by fl uorescent in situ hybridization. During the early passages, monosomal cells were more often detected than trisomal ones. A clone with chromosome 6 monosomy was detected in three cultures during late passages.
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