Journal
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
Volume 91, Issue 6, Pages 635-639Publisher
SPRINGER
DOI: 10.1007/s00128-013-1101-9
Keywords
Di-n-butyl phthalate; Diethyl phthalate; Acetylcholinesterase; Neurotoxicity related gene expression; Zebrafish
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Funding
- Research Foundation for Advanced Talents of Jiangsu University [13JDG014]
- Specialized Research Fund for the Doctoral Program of Chinese Universities from Ministry of Education [20113227110020]
- Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology [HCK201017]
- State key laboratory of Environmental Chemistry and Ecotoxicology [KF2011-20]
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In the present study, zebrafish embryos were used to assess the neurotoxicity of di-n-butyl phthalate (DBP), diethyl phthalate (DEP) and their mixture. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to various concentrations of DBP, DEP and their mixture (DBP-DEP) until 96 hpf. The transcriptions levels of selected neuron-related genes reported as neurotoxicity biomarkers were analyzed. The results showed that transcripts of growth associated protein 43 (gap43), embryonic lethal abnormal vision-like 3 (elavl3), glial fibrillary acidic protein (gfap), myelin basic protein (mbp), alpha 1-tubulin and neurogenin1 (ngn1) were significantly up-regulated after DBP, DEP and DBP-DEP mixture exposure. In addition, acetylcholinesterase activity was significantly inhibited in the embryos. These results indicate that DBP and DEP have the potential neurotoxicity in zebrafish embryos.
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