Journal
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
Volume 83, Issue 2, Pages 155-158Publisher
SPRINGER
DOI: 10.1007/s00128-009-9771-z
Keywords
Comet assay; Prochilodus lineatus; Genotoxic effects; Cypermethrin
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Funding
- Universidad Nacional del Litoral, Argentina [PI CAI + D Ndegrees 21/123 2006]
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Toxicants on fish may induce genetic alterations that can be used as genotoxic markers. We evaluated DNA damage using alkaline comet assay applied on erythrocytes after in vivo exposure of Prochilodus lineatus to different concentrations of Cypermethrin (0.300, 0.150, 0.075 and 0.000 mu g/L) as a probable chemical mutagen. The results revealed a significantly higher level of DNA damage at all concentrations of Cypermethrin tested compared to control and background level (p < 0.05). We have standardized the technique for one of the most common native fish species that will be useful for biomonitoring genotoxicity in polluted waters of the region.
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