Journal
BULLETIN OF ENTOMOLOGICAL RESEARCH
Volume 101, Issue 1, Pages 45-52Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007485310000167
Keywords
Rhagoletis cerasi; Tephritidae; microsatellites; cross-species amplification; population analysis
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Funding
- European Community [CSA-SA_REGPROT-2007-1, 203590]
- University of Ioannina
- European Social and National Resources - EPEAEK II - Pythagoras
- Greek ministry of education
- University of Thesssaly
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The European cherry fruit fly, Rhagoletis cerasi (Diptera: Tephritidae), is a major pest of cherries in Europe and parts of Asia. Despite its big economic significance, there is a lack of studies on the genetic structure of its natural populations. Knowledge about an insect pest on molecular, genetic and population levels facilitates the development of environmentally friendly control methods. In this study, we present the development of 13 microsatellite markers for R. cerasi, through cross-species amplification. These markers have been used for the genotyping of 130 individuals from five different sampling sites in Greece. Our results indicate that (i) cross-species amplification is a versatile and rapid tool for developing microsatellite markers in Rhagoletis spp., (ii) the microsatellite markers presented here constitute an important tool for population studies on this pest, and (iii) there is clear structuring of natural European cherry fly populations.
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