Journal
BULLETIN OF ENTOMOLOGICAL RESEARCH
Volume 98, Issue 3, Pages 233-238Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007485308005981
Keywords
Jasus edwardsii; Haliotis rubra; polymerase chain reaction (PCR); prey detection; rock lobster diet
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We demonstrate the use of molecular techniques to detect specific prey consumed by the southern rock lobster (Jasus edwardsii). A quick and non-lethal method was used to collect rock lobster faecal material and a molecular protocol was employed to isolate prey DNA from faecal samples. The isolated DNA was amplified using the polymerase chain reaction (PCR) with PCR primers designed to target specific prey items. Feeding experiments determined that DNA from black-lipped abalone (Haliotis rubra) and sea urchins (Centrostephanus rodgersii and Heliocidaris erythrogramma) can be detected in rock lobster faecal samples within seven hours and remains present for up to 60h after ingestion.
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