Journal
CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
Volume 41, Issue 3, Pages 179-188Publisher
IOS PRESS
DOI: 10.3233/CH-2009-1168
Keywords
Erythrocyte deformability; erythrocyte aggregation; oxygenation; deoxygenation
Categories
Funding
- NIH [HL15722, HL 70595, IR03 TW01295]
- Akdeniz University Research Projects Unit
- FOGARTY INTERNATIONAL CENTER [R03TW001295] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL015722, U54HL070595, R37HL015722] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Measurements of red blood cell (RBC) deformability and aggregation can be subject to influence by pre-analytical handling procedures, with the degree of hemoglobin oxygenation having the potential to affect the results. To examine such effects, RBC deformability and aggregation were studied before and after oxygenation or deoxygenation of human blood samples. RBC deformability was assessed using a laser-diffraction ektacytometer having Couette geometry. RBC aggregation was assessed using the same system by monitoring light backscattering after a sudden cessation of high shear; aggregation was also measured by monitoring light transmittance through RBC suspensions. RBC deformability was found to be significantly increased after equilibrating RBC with ambient air (pO(2): 142.0 +/- 3.1 mmHg) compared to the non-oxygenated sample (pO(2): 42.4 +/- 1.8 mmHg). In contrast, equilibration with 100% nitrogen resulted in significant impairment in RBC deformability. RBC aggregation parameters were also affected by oxygenation if measured based on light backscattering, but not if measured using light transmittance. It is thus recommended that blood samples be oxygenated by repeated exposure to ambient air prior to the measurement of hemorheological parameters.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available