Journal
JOURNAL OF CONTROLLED RELEASE
Volume 217, Issue -, Pages 337-344Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2015.08.051
Keywords
mRNA; Modified RNA; Nucleobase modifications; Antiviral innate immunity; Toll-like receptor; Gene therapy
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Messenger RNA as a therapeutic modality is becoming increasingly popular in the field of gene therapy. The realization that nucleobase modifications can greatly enhance the properties of mRNA by reducing the immunogenicity and increasing the stability of the RNA molecule (the Kariko paradigm) has been pivotal for this revolution. Here we find that mRNAs containing the N-1-methylpseudouridine (m1 Psi) modification alone and/or in combination with 5-methylcytidine (m5C) outperformed the current state-of-the-art pseudouridine (Psi) and/or m5C/Psi-modified mRNA platform by providing up to similar to 44-fold (when comparing double modified mRNAs) or similar to 13-fold (when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lines or mice, respectively. We show that (m5C/) m1 Psi-modified mRNA resulted in reduced intracellular innate immunogenicity and improved cellular viability compared to (m5C/)Psi-modified mRNA upon in vitro transfection. The enhanced capability of (m5C/) m1 Psi-modified mRNA to express proteins may at least partially be due to the increased ability of the mRNA to evade activation of endosomal Toll-like receptor 3 (TLR3) and downstream innate immune signaling. We believe that the (m5C/) m1 Psi-mRNA platform presented here may serve as a new standard in the field of modified mRNA-based therapeutics. (c) 2015 Elsevier B.V. All rights reserved.
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