Journal
BRITISH JOURNAL OF PHARMACOLOGY
Volume 162, Issue 1, Pages 147-162Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1476-5381.2010.01020.x
Keywords
inflammation; prostaglandin; boswellic acid; microsomal prostaglandin E-2 synthase; arachidonic acid
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Funding
- Pharmasan GmbH (Freiburg, Germany)
- Medeon GmbH, Berlin, Aureliasan GmbH (Tuebingen, Germany)
- Deutsche Forschungsgemeinschaft
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BACKGROUND AND PURPOSE Frankincense, the gum resin derived from Boswellia species, showed anti-inflammatory efficacy in animal models and in pilot clinical studies. Boswellic acids (BAs) are assumed to be responsible for these effects but their anti-inflammatory efficacy in vivo and their molecular modes of action are incompletely understood. EXPERIMENTAL APPROACH A protein fishing approach using immobilized BA and surface plasmon resonance (SPR) spectroscopy were used to reveal microsomal prostaglandin E-2 synthase-1 (mPGES1) as a BA-interacting protein. Cell-free and cell-based assays were applied to confirm the functional interference of BAs with mPGES1. Carrageenan-induced mouse paw oedema and rat pleurisy models were utilized to demonstrate the efficacy of defined BAs in vivo. KEY RESULTS Human mPGES1 from A549 cells or in vitro-translated human enzyme selectively bound to BA affinity matrices and SPR spectroscopy confirmed these interactions. BAs reversibly suppressed the transformation of prostaglandin (PG)H-2 to PGE(2) mediated by mPGES1 (IC50 = 3-10 mu M). Also, in intact A549 cells, BAs selectively inhibited PGE(2) generation and, in human whole blood, beta-BA reduced lipopolysaccharide-induced PGE(2) biosynthesis without affecting formation of the COX-derived metabolites 6-keto PGF(1 alpha) and thromboxane B-2. Intraperitoneal or oral administration of beta-BA (1 mg center dot kg-1) suppressed rat pleurisy, accompanied by impaired levels of PGE(2) and beta-BA (1 mg center dot kg-1, given i.p.) also reduced mouse paw oedema, both induced by carrageenan. CONCLUSIONS AND IMPLICATIONS Suppression of PGE(2) formation by BAs via interference with mPGES1 contribute to the anti-inflammatory effectiveness of BAs and of frankincense, and may constitute a biochemical basis for their anti-inflammatory properties.
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