Journal
BRITISH JOURNAL OF PHARMACOLOGY
Volume 150, Issue 4, Pages 445-454Publisher
WILEY
DOI: 10.1038/sj.bjp.0706994
Keywords
polymyxin B; extracellular ATP; P2 receptors; P2X(7); endotoxin
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Funding
- Telethon [GGP06070] Funding Source: Medline
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Background and purpose: P2X(7) is a membrane receptor for extracellular ATP which is highly expressed in dendritic cells, macrophages and microglia where it mediates pro-inflammatory responses. The antibiotic polymyxin B, which binds to and neutralizes the toxic residue of bacterial lipopolysaccharide, greatly amplifies cellular responses mediated by the P2X7 receptor. However, the molecular mechanism involved is so far unknown. Experimental approach: We investigated the effects of polymyxin B and polymyxin B nonapeptide (PMBN) which is the deacylated amino derivative of polymyxin B lacking the N-terminal fatty amino acid 6-methylheptanoic/ octanoic-Dab residue, in human macrophages and HEK293 cells stably expressing the human P2X(7) receptor (HEK293-hP2X(7)). Differences between the two antibiotics were assessed by monitoring the following: nucleotide-induced cytoplasmic free Ca2+ concentration changes, plasma membrane permeability changes, lactate dehydrogenase activity, cell morphology changes. Western blot and microscopic analyses of P2X(7)GFP-expressing cells were also performed. Key results: In contrast to polymyxin B, the polymyxin B nonapeptide was unable to potentiate: a) the ATP-induced Ca2+ increase, b) pore formation and consequently ATP-mediated plasma membrane permeabilization; c) ATP-dependent cytotoxicity. Moreover, in contrast to polymyxin B, polymyxin B nonapeptide did not affect aggregation of the P2X7 receptor subunits and it did not potentiate P2X(7)-dependent cell fusion. Conclusions and Implications: The effects of polymyxin B depended on the presence of its N-terminal fatty amino acid 6-methylheptanoic/octanoic-Dab residue as deletion of this residue abolished polymyxin B-dependent modulation of ATP-triggered responses. These findings are important in the search for allosteric modulators of the P2X7 receptor.
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