Journal
JOURNAL OF CONTROLLED RELEASE
Volume 209, Issue -, Pages 186-196Publisher
ELSEVIER
DOI: 10.1016/j.jconrel.2015.04.020
Keywords
Multifunctional nanoparticles; Manganese oxide; Cancer; Dual modal imaging; Drug delivery
Funding
- Natural Science and Engineering Research Council Canada [RGPIN 170460-13]
- University of Toronto Scholarship
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Multifunctional nanoparticles (NPs) have found important applications in diagnosis, chemotherapy, and image-guided surgery of tumors. In this work, we have developed polymeric theranostic NPs (PTNPs) containing the anticancer drug docetaxel (DTX), a fluorescent dye, and magnetic manganese oxide (MnO) NPs for dual modal imaging and chemotherapy. PTNPs similar to 150 nm in diameter were synthesized by co-loading hydrophobic DTX and MnO NPs similar to 5 nm in diameter, into the matrix of a fluorescent dye-labeled amphiphilic polymer. The PTNPs enabled high loading efficiency and sustained in vitro release of DTX. Energy-dependent cellular uptake and extended cytoplasmic retention of the PTNPs in MDA-MB-231 human breast cancer cells were observed by fluorescence microscopy examination. DTX-loaded PTNPs exhibited higher cytotoxicity than free DTX with a 3 to 4.4-fold decrease in drug dose required for 50% cell growth inhibition. The hydrophilic backbone of the amphiphilic polymer improved the fluidity of PTNPs which enhanced the longitudinal relaxivity (r(1)) of loaded MnO NPs by 2.7-fold with r(1) = 2.4 mM(-1) s(-1). Whole body fluorescence imaging (FI) and magnetic resonance imaging (MRI) showed significant accumulation and prolonged retention of PTNPs in orthotopic MDA-MB-231 breast tumors. These results suggest that the newamphiphilic polymer-based PTNP system, able to simultaneously deliver a poorly soluble anticancer drug, enhance MRI contrast, and stain tumor tissue by fluorescence, is a good candidate for cancer theranostic applications. (C) 2015 Elsevier B.V. All rights reserved.
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