Fruit juice drinks prevent endogenous antioxidant response to high-fat meal ingestion

High-fat meals (HFM) induce metabolic stress, leading to the activation of protective mechanisms, including inflammation and endogenous antioxidant defences. In the present study, we investigated the effects of antioxidant-rich fruit juice drinks on the endogenous antioxidant response induced by HFM. In a double-blind, cross-over design (10d washout), fourteen overweight volunteers were randomly assigned to one of the following interventions: HFM+500ml placebo beverage (HFM-PB, free from fruit); HFM+500ml antioxidant beverage 1 (HFM-AB1; apple, grape, blueberry and pomegranate juices and grape skin, grape seed and green tea extracts); HFM+500ml antioxidant beverage 2 (HFM-AB2; pineapple, black currant and plum juices). HFM-PB consumption increased the plasma levels of thiols (SH) (4h, P<0 center dot 001) and uric acid (UA) (2h, P<0 center dot 01) and total radical-trapping antioxidant parameter (TRAP) (4h, P<0 center dot 01). Following the consumption of drinks, UA production was significantly reduced with respect to placebo beverage consumption 8h after HFM-AB2 consumption (P<0 center dot 05). SH levels were reduced 0 center dot 5 (P<0 center dot 05), 1 (P<0 center dot 05) and 2h (P<0 center dot 01) after HFM-AB1 consumption and 2, 4 and 8h (P<0 center dot 05) after HFM-AB2 consumption. Plasma TRAP (2h, P<0 center dot 001) and urinary ferric reducing antioxidant power (0-8h, P<0 center dot 01) were increased by HFM-AB1 consumption, the drink with the highest in vitro antioxidant capacity, but not by HFM-AB2 consumption. In urine, UA levels were significantly increased from basal levels after the consumption of HFM-PB and HFM-AB2. However, neither of the beverages increased the urinary excretion of UA with respect to the placebo beverage. In conclusion, the increase in UA and SH levels induced by HFM as part of an endogenous antioxidant response to postprandial stress can be prevented by the concomitant ingestion of antioxidant-rich fruit juice drinks.