Journal
BRITISH JOURNAL OF NUTRITION
Volume 104, Issue 1, Pages 17-23Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114510000309
Keywords
Selenium; Selenoproteins; Colon cancer prevention
Categories
Funding
- Gardiner Foundation Major Research and Development Project (Melbourne, VIC, Australia) [MP4/009]
- Department of Primary Industries Victoria, Tatura Milk Industries Ltd (Tatura, VIC, Australia)
- Alltech Biotech Pty Ltd (Dandenong South, Vic. Australia)
- Geoffrey Gardiner Foundation (Melbourne, VIC, Australia)
- National Health and Medical Research Council of Australia
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Certain forms of dietary Se may have an advantage in improving Se status and reducing cancer risk. The present study compared the effects of an Se-enriched milk protein product (dairy-Se) with an Se yeast (yeast-Se) on selenoprotein activity and expression in the mouse colon. Mice were fed four diets for 4 weeks: a control milk protein diet (Se at 0.068 parts per million (ppm)), dairy-Se diets with Se at 0.5 and 1 ppm, and a yeast-Se diet with Se at 1 ppm. Cytosolic glutathione peroxidase-1 (GPx-1) activity, mRNA of selenoprotein P (SeP), GPx-1, gastrointestinal glutathione peroxidase-2 (GPx-2) and thioredoxin reductase-1 (Trx-R-1) were examined in the mouse colon. Dairy-Se diets did not significantly affect GPx-I mRNA and GPx-1 activity but produced a dose-dependent increase in SeP and GPx-2 mRNA, with a significantly higher level achieved at 1 ppm Se (P<0.05). Yeast-Se at 1 ppm significantly increased GPx-I mRNA and GPx-1 activity (P<0.01) but not GPx-2 mRNA. Neither Se supplement had any effect on TrxR-1. The present study indicates that selenoprotein levels in the mouse colon are regulated differently depending on the Se supplement. As we have previously shown that dairy-Se at 1 ppm was protective against colorectal cancer (CRC) in an azoxymethane-induced CRC mouse model, this up-regulation of colonic GPx-2 and SeP with Se supplementation may be crucial to its chemopreventive action.
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