Journal
BRITISH JOURNAL OF NUTRITION
Volume 102, Issue 4, Pages 554-562Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114509220812
Keywords
Blackcurrants; Tocopherol; Comet assay; Antioxidant capacity in stool
Categories
Funding
- Federal Ministry of Education and Research
Ask authors/readers for more resources
Berry seeds are a tocopherol-rich by-product of fruit processing without specific commercial value. In a human intervention study, the physiological impact of blackcurrant seed press residue (PR) was tested. Thirty-six women (aged 24 +/- 3 years; twenty non-smokers, sixteen smokers) consumed 250g bread/d containing 8% PR for a period of 4 weeks (period 3). Comparatively, a control bread without PR (250g/d) was tested (period 2) and baseline data were obtained (period 1). Blood, stool and 24h urine were collected during a 5d standardised diet within each period. Tocopherol and Fe intakes were calculated from food intake. In serum, tocopherol concentration and Fe parameters were determined. In urine, oxidative stress markers 8-oxo-2'-deoxyguanosine, 8-iso-PGF(2 alpha) and inflammatory response marker 15-keto-dihydro-PGF(2 alpha) were analysed. Stool tocopherol concentration, genotoxicity of faecal water (comet assay) and antioxidant capacity of stool (aromatic hydroxylation of salicylic acid) were determined. Fe and total tocopherol intake, total tocopherol concentrations in serum and stool, and genotoxicity of faecal water increased with PR bread consumption (P<0.05). The antioxidant capacity of stool decreased between baseline and intervention, expressed by increased formation of 2,3- and 2,5-dihydroxybenzoic acid in vitro (P<0.05). In smokers, 8-oxo-2'-deoxyguanosine increased with PR consumption (P<0.05). Prostane concentrations were unaffected by PR bread consumption. In summary, the intake of bread containing blackcurrant PR for 4 weeks increased serum and stool total tocopherol concentrations. However, various biomarkers indicated increased oxidative stress, suggesting that consumption of ground berry seed may not be of advantage.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available