Absorption and pharmacokinetics of green tea catechins in beagles

The present study evaluates for the first time in dogs, the kinetics of green tea catechins and their metabolic forms in plasma and urine. Ten beagles were administered 173 mg (12.35 mg/kg body weight) of catechins as a green tea extract, in capsules. Blood samples were collected during 24h after intake and urine samples were collected during the following time: 0-2, 2-6, 6-8 and 8-24h. Two catechins with a galloyl moiety and around 1h. Median C-max for (-)-epigallocatechin-3-gallate (EGCG), (-)-epicatechin-3-gallate (ECG), (-)-epigallocatechin glucuronide (EGC-glucuronide), (-)-epicatechin glucuronide (EC-glucuronide), (-)-epicatechin sulphate (EC-sulphate) were 0.3 (range 0.1-1.9), 0.1 (range 0-0.4), 0.8 (range 0.2-3.9), 0.2 (range 0.1-1.7) and 1 (range 0.3-3.4)mu mol/l, respectively. The areas under the plasma concentration v. time curves (AUC(0 -> 24)) mu mol/l x min for EGCG, 40 (range 12-258) mu mol/l x min for EC-glucuronide and 14 (range 0.1-124) mu mol/l x min for ECG. The values of mean residence time (MRT0 -> 24) were 5 (range 2-16), 2 (range 1-11), 10 (range 2-13), 3 (range 2-16) and 2.4 (range 1-18) h for EGCG, eCG, EGC-glucuronide, EC-glucuronide and EC-sulphate, respectively. In urine, catechins were present as conjugated forms, suggesting bile excretion of EGCG and ECG. Green tea catechins are absorbed following an oral administration and EGC-glucuronide is the metabolic form that remains in the organism for a longer period of time, suggesting that this compound could suffer an enterohepatic cycle.