Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 4, Pages 2290-2298Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac504032d
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Funding
- Spanish Ministerio de Economia y Competitividad Research (NANOAVANSENS Program from the Comunidad de Madrid) [CTQ2011-24355, CTQ2012-34238, S2013/MT-3029]
- Spanish Ministerio de Ciencia e Innovacion
- Spanish Ministry of Science and Innovation
- Ramon y Cajal Programme of the MINECO
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Novel disposable electrochemical DNA sensors were prepared for the detection of a target DNA sequence on the p53 tumor suppressor (TP53) gene. The electrochemical platform consisted of screen-printed carbon electrodes (SPCEs) functionalized with a water-soluble reduced graphene oxidecarboxymethylcellulose (rGO-CMC) hybrid nanomaterial. Two different configurations involving hairpin specific capture probes of different length covalently immobilized through carbodiimide chemistry on the surface of rGO-CMC-modified SPCEs were implemented and compared. Upon hybridization, a streptavidin-peroxidase (Strep-HRP) conjugate was employed as an electrochemical indicator. Hybridization was monitored by recording the amperometric responses measured at -0.10 V (vs an Ag pseudo-reference electrode) upon the addition of 3,3',5,5'-tetramethylbenzidine (TMB) as a redox mediator and H2O2 as an enzyme substrate. The implemented DNA platforms allow single nucleotide polymorphism (SNP) discrimination in cDNAs from human breast cancer cell lines, which makes such platforms excellent as new diagnosis tools in clinical analysis.
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