Journal
BRITISH JOURNAL OF CANCER
Volume 111, Issue 5, Pages 909-917Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/bjc.2014.360
Keywords
blood serum; cancer-testis antigens; circulating microRNAs; circulating RNA; overall survival
Categories
Funding
- Stiftung fur Pathochemie und Molekulare Diagnostik
- Karlsruhe/Hamburg
- ERC (European Research Council) [ERC-2010 AsG 20100317 DISSECT]
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Background: MAGE-A (melanoma-associated antigen-A) are promising targets for specific immunotherapy and their expression may be induced by the epigenetic factor BORIS. Methods: To determine their relevance for breast cancer, we quantified the levels of MAGE-A1, -A2, -A3, -A12 and BORIS mRNA, as well as microRNAs let-7b and miR-202 in pre- and postoperative serum of 102 and 34 breast cancer patients, respectively, and in serum of 26 patients with benign breast diseases and 37 healthy women by real-time PCR. The mean follow-up time of the cancer patients was 6.2 years. Results: The serum levels of MAGE-A and BORIS mRNA, as well as let-7b were significantly higher in patients with invasive carcinomas than in patients with benign breast diseases or healthy women (P<0.001), whereas the levels of miR-202 were elevated in both patient cohorts (P<0.001). In uni-and multivariate analyses, high levels of miR-202 significantly correlated with poor overall survival (P = 0.0001). Transfection of breast cancer cells with synthetic microRNAs and their inhibitors showed that let-7b and miR-202 did not affect the protein expression of MAGE-A1. Conclusions: Based on their cancer-specific increase in breast cancer patients, circulating MAGE-A and BORIS mRNAs may be further explored for early detection of breast cancer and monitoring of MAGE-directed immunotherapies. Moreover, serum miR-202 is associated with prognosis.
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