Journal
BRITISH JOURNAL OF CANCER
Volume 107, Issue 2, Pages 352-359Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/bjc.2012.251
Keywords
PRL-3; microRNA; STAT3; colon carcinoma
Categories
Funding
- National Natural Science Foundation of China [81071761]
- Natural Science Foundation of Guangdong Province [10151008901000044]
- Medical Science Research Foundation of Guangdong Province [A2011171]
Ask authors/readers for more resources
BACKGROUND: Phosphatase of regenerating liver-3 (PRL-3) is an oncogene known to promote tumour metastasis, especially in colorectal cancer (CRC). Here, we demonstrate that the miR-21, miR-17 and miR-19a expressions induced by PRL-3 are involved in the proliferation and metastasis of colon cancer. METHODS: Microarray analysis and quantitative reverse-transcription polymerase chain reactions (qRT-PCR) were used to investigate the changes in miRNA expression due to the overexpression of PRL-3. Transwell chamber invasion assays, CCK-8 proliferation assays and RNA interference assays were used to explore the effects of PRL-3 on miR-21, miR-17 and miR-19a expression in colon cancer cells. Immunohistochemistry and qRT-PCR were performed in colon cancer tissues to evaluate the expression of PRL-3, signal transducer and activator of transcription 3 (STAT3), miR-21, miR-17 and miR-19a. RESULTS: Our study demonstrated that the overexpression of PRL-3 in colon cancer cells induced the expression of miR-21, miR-17 and miR-19a by activating STAT3. Subsequently, these microRNAs contributed to the increased proliferation and invasiveness of the colon cancer cells. Positive correlations between PRL-3 and these microRNAs were also observed in matched primary colon cancer tissues and metastatic lesions. CONCLUSION: miR-21, miR-17 and miR-19a induced by PRL-3 contribute to the proliferation and invasion of colon cancer. British Journal of Cancer (2012) 107, 352-359. doi:10.1038/bjc.2012.251 www.bjcancer.com Published online 7 June 2012 (c) 2012 Cancer Research UK
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available