Journal
BRITISH JOURNAL OF CANCER
Volume 107, Issue 1, Pages 91-99Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/bjc.2012.217
Keywords
gene expression profiling; microarray; ovarian serous adenocarcinoma; GRIA2; survival
Categories
Funding
- Ministry for Health, Welfare and Family affairs, Republic of Korea [0920010, A092255]
Ask authors/readers for more resources
BACKGROUND: The purpose of this study was to identify genes that are differentially expressed in chemosensitive serous papillary ovarian carcinomas relative to those expressed in chemoresistant tumours. METHODS: To identify novel candidate biomarkers, differences in gene expression were analysed in 26 stage IIIC/IV serous ovarian adenocarcinomas (12 chemosensitive tumours and 14 chemoresistant tumours). We subsequently investigated the immunohistochemical expression of GRIA2 in 48 independent sets of advanced ovarian serous carcinomas. RESULTS: Microarray analysis revealed a total of 57 genes that were differentially expressed in chemoresistant and chemosensitive tumours. Of the 57 genes, 39 genes were upregulated and 18 genes were downregulated in chemosensitive tumours. Five differentially expressed genes (CD36, LIFR, CHL1, GRIA2, and FCGBP) were validated by quantitative real-time PCR. The expression of GRIA2 was validated at the protein level by immunohistochemistry, and patients with GRIA2 expression showed a longer progression-free and overall survival (P = 0.051 and P = 0.031 respectively). CONCLUSIONS: We found 57 differentially expressed genes to distinguish between chemosensitive and chemoresistant tumours. We also demonstrated that the expression of GRIA2 among the differentially expressed genes provides better prognosis of patients with advanced serous papillary ovarian adenocarcinoma. British Journal of Cancer (2012) 107, 91-99. doi:10.1038/bjc.2012.217 www.bjcancer.com Published online 29 May 2012 (C) 2012 Cancer Research UK
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available