Journal
BRITISH JOURNAL OF CANCER
Volume 104, Issue 9, Pages 1410-1417Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/bjc.2011.94
Keywords
cancer stem cells; side population; non-small cell lung tumour cells; transcription factor; SOX2; Hoechst 33342 dye
Categories
Funding
- National Natural Science Foundation of China (NSFC) [30671938, 81071711, 91029734]
- NIH [CA 115751]
- EMD Serono Research Center [SFP 1645]
- [30830096]
- [2007CB914804]
- [09ZCZDSF04000]
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BACKGROUND: The cancer stem cell hypothesis suggests that neoplastic clones are maintained exclusively by a small subpopulation of cells, which have indefinite proliferation and differentiation potentials and give rise to phenotypically diverse cancer cells. Cancer stem cells have been isolated by their ability to efflux Hoechst 33342 dye and are referred to as the 'side population' (SP). METHODS AND RESULTS: The Hoechst efflux assay was used to isolate and characterize the SP from murine D121 lung carcinoma cells. Here, we demonstrated that D121-SP cells contain cancer stem cell characteristics, that is, upregulation of the transcription factors SOX2 and Oct 4 in D121-SP cells. In addition, the migration of D121-SP was decreased, and apoptosis of D121-SP was upregulated following knocking down of SOX2 in D121 cells. Importantly, downregulation of SOX2 in D121 cells markedly suppressed their metastatic potential in syngeneic mice. CONCLUSIONS: These results suggest that the SP is an enriched source of lung tumour cells with stem cell properties and that SOX2 has an important role in maintaining stem cell properties and functions that may be a potential target for effective lung cancer therapy. British Journal of Cancer (2011) 104, 1410-1417. doi:10.1038/bjc.2011.94 www.bjcancer.com Published online 5 April 2011 (C) 2011 Cancer Research UK
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