Journal
BRITISH JOURNAL OF CANCER
Volume 102, Issue 10, Pages 1495-1502Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.bjc.6605676
Keywords
circulating tumour cells; breast cancer; lung cancer; HER2; FISH
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Funding
- National Cancer Institute Lung [SPORE P50CA090578]
- Department of Defense [W81XWH06-1-0303]
- twoAM fund (IEK)
- Hazel and Samuel Bellin research fund
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BACKGROUND: Circulating tumour cells (CTCs) offer a non-invasive approach to obtain and characterise metastatic tumour cells, but their usefulness has been limited by low CTC yields from conventional isolation methods. METHODS: To improve CTC yields and facilitate their molecular characterisation we compared the Food and Drug Administration-approved CellSearch Epithelial Kit (CEK) to a simplified CTC capture method, CellSearch Profile Kit (CPK), on paired blood samples from patients with metastatic breast (n=75) and lung (n=71) cancer. Molecular markers including Human Epidermal growth factor Receptor 2 (HER2) were evaluated on CTCs by fluorescence in situ hybridisation (FISH) and compared to patients' primary and metastatic cancer. RESULTS: The median cell count from patients with breast cancer using the CPK was 117 vs 4 for CEK (P<0.0001). Lung cancer samples were similar; CPK: 145 cells vs CEK: 4 cells (P<0.0001). Recovered CTCs were relatively pure (60-70%) and were evaluable by FISH and immunofluorescence. A total of 10 of 30 (33%) breast cancer patients with HER2-negative primary and metastatic tissue had HER2-amplified CTCs. CONCLUSION: The CPK method provides a high yield of relatively pure CTCs, facilitating their molecular characterisation. Circulating tumour cells obtained using CPK technology demonstrate that significant discordance exists between HER2 amplification of a patient's CTCs and that of the primary and metastatic tumour. British Journal of Cancer (2010) 102, 1495-1502. doi:10.1038/sj.bjc.6605676 www.bjcancer.com (C) 2010 Cancer Research UK
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