Journal
BRITISH JOURNAL OF CANCER
Volume 101, Issue 2, Pages 320-326Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.bjc.6605143
Keywords
colorectal cancer; cancer stem cell; integrin; epithelial-mesenchymal transition; collagen; CD49b
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Funding
- Wellcome Trust [060688]
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BACKGROUND: Human colorectal cancer is caused by mutations and is thought to be maintained by a population of cancer stem cells. Further phenotypic changes occurring at the invasive edge suggest that colon cancer cells are also regulated by their microenvironment. Type I collagen, a promoter of the malignant phenotype in pancreatic carcinoma cells, is highly expressed at the invasive front of human colorectal cancer. METHODS: This study investigates the role of type I collagen in specifying the colorectal cancer cell phenotype. The effect of type I collagen on morphology, localisation of cell-cell adhesion proteins, differentiation and stem cell-like characteristics was examined in a panel of human colorectal carcinoma cell lines. RESULTS: Human colorectal carcinoma cells grown on type I collagen in serum-free medium show an epithelial-mesenchymal-like transition (EMT-like), assuming a more flattened less cohesive morphology. Type I collagen downregulates E-cadherin and beta-catenin at cell-cell junctions. Furthermore, type I collagen inhibits differentiation, increases clonogenicity and promotes expression of stem cell markers CD133 and Bmi1. Type I collagen effects were partially abrogated by a function-blocking antibody to alpha 2 integrin. CONCLUSION: Together, these results indicate that type I collagen promotes expression of a stem cell-like phenotype in human colorectal cancer cells likely through alpha 2 beta 1 integrin. British Journal of Cancer ( 2009) 101, 320-326. doi:10.1038/sj.bjc.6605143 www.bjcancer.com Published online 30 June 2009 (C) 2009 Cancer Research UK
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