Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 23, Pages 11714-11720Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b02654
Keywords
-
Categories
Funding
- NIH [GM079359, CA133086]
- National Key Scientific Program of China [2011CB911000]
- Foundation for Innovative Research Groups of the NSFC [21221003]
- China National Instrumentation Program [2011YQ03012412]
Ask authors/readers for more resources
Here, we propose an efficient strategy for enzyme- and hairpin-free nucleic acid detection called an entropy beacon (abbreviated as Ebeacon). Different from previously reported DNA hybridization/displacement-based strategies, Ebeacon is driven forward by increases in the entropy of the system, instead of free energy released from new base-pair formation. Ebeacon shows high sensitivity, with a detection limit of 5 pM target DNA in buffer and 50 pM in cellular homogenate. Ebeacon also benefits from the hairpin-free amplification strategy and zero-background, excellent thermostability from 20 degrees C to 50 degrees C, as well as good resistance to complex environments. In particular, based on the huge difference between the breathing rate of a single base pair and two adjacent base pairs, Ebeacon also shows high selectivity toward base mutations, such as substitution, insertion, and deletion and, therefore, is an efficient nucleic acid detection method, comparable to most reported enzyme-free strategies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available