Journal
BRIEFINGS IN BIOINFORMATICS
Volume 14, Issue 3, Pages 344-360Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bib/bbs041
Keywords
polo-like kinase; phospho-binding; phosphorylation; PBD; GPS
Funding
- National Basic Research Program (973 project) [2012CB911201, 2012CB910101, 2010CB945401, 2010CB912103, 2011CB910400]
- Natural Science Foundation of China [90508002, 90913016, 31071154, 30900835, 30830036, 91019020, 31171300, 31171263, 21075045]
- Chinese Academy of Sciences [KSCX1-YW-R-65, KSCX2-YW-H-10, KSCX2-YW-R-195, KSCX2-YW-R-140]
- Fundamental Research Funds for the Central Universities [HUST: 2011TS085, SYSU: 11lgzd11]
- Anhui Province Key Project [08040102005]
Ask authors/readers for more resources
Substantial evidence has confirmed that Polo-like kinases (Plks) play a crucial role in a variety of cellular processes via phosphorylation-mediated signaling transduction. Identification of Plk phospho-binding proteins and phosphorylation substrates is fundamental for elucidating the molecular mechanisms of Plks. Here, we present an integrative approach for the analysis of Plk-specific phospho-binding and phosphorylation sites (p-sites) in proteins. From the currently available phosphoproteomic data, we predicted tens of thousands of potential Plk phospho-binding and phosphorylation sites in eukaryotes, respectively. Furthermore, statistical analysis suggested that Plk phospho-binding proteins are more closely implicated in mitosis than their phosphorylation substrates. Additional computational analysis together with in vitro and in vivo experimental assays demonstrated that human Mis18B is a novel interacting partner of Plk1, while pT14 and pS48 of Mis18B were identified as phospho-binding sites. Taken together, this systematic analysis provides a global landscape of the complexity and diversity of potential Plk-mediated phosphoregulation, and the prediction results can be helpful for further experimental investigation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available