Journal
BREEDING SCIENCE
Volume 61, Issue 5, Pages 480-494Publisher
JAPANESE SOC BREEDING
DOI: 10.1270/jsbbs.61.480
Keywords
Soybean [Glycine max (L.) Merrill]; transformation; Agrobacterium tumefaciens; particle bombardment
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Ministry of Agriculture, Forestry and Fisheries of Japan [GMZ-1004]
- TRANSNET
- Grants-in-Aid for Scientific Research [23580002] Funding Source: KAKEN
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Herbicide-resistant transgenic soybean plants hold a leading market share in the USA and other countries, but soybean has been regarded as recalcitrant to transformation for many years. The cumulative and, at times, exponential advances in genetic manipulation have made possible further choices for soybean transformation. The most widely and routinely used transformation systems are cotyledonary node-Agrobacterium-mediated transformation and somatic embryo-particle-bombardment-mediated transformation. These ready systems enable us to improve seed qualities and agronomic characteristics by transgenic approaches. In addition, with the accumulation of soybean genomic resources, convenient or promising approaches will be requisite for the determination and use of gene function in soybean. In this article, we describe recent advances in and problems of soybean transformation, and survey the current transgenic approaches for applied and basic research in Japan.
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