Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 18, Pages 9288-9293Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b01923
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Funding
- Natural Sciences and Engineering Research Council of Canada
- Canadian Foundation for Innovation
- Ontario Ministry of Research and Innovation
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In this study, a paper-based point-of-care (POC) colorimetric biosensor was developed for the detection of lactate dehydrogenase in serum using a nonporous, oxygen impermeable reversibly gelling polysaccharide material based on pullulan. The pullulan could be printed onto paper surfaces along with all required assay reagents, providing a means for high-stability immobilization of all reagents on paper. Serum containing lactate dehydrogenase (LDH) was directly spotted on to the pullulan-coated bioactive paper and provided quantitative colorimetric data that was comparable to that obtained with a conventional plate-reader method. The paper strip was found to be highly stable and could be stored at 4 degrees C for at least 10 weeks with no loss in performance, as compared to a complete loss in performance within 1 day when the reagents were printed without the stabilizing polysaccharide. The ease of fabrication coupled with the high stability of the printed reagents provides a facile platform for easily manufactured POC sensors.
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