Circulating levels of inflammatory markers and mammographic density among postmenopausal women

Mammographic density is strongly associated with breast cancer risk. Inflammation is involved in breast carcinogenesis, perhaps through effects on mammographic density. We evaluated associations between inflammatory markers interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and C-reactive protein (CRP) and mammographic density among postmenopausal women. Plasma IL-6, TNF-alpha, and CRP levels were measured in 145 women with benign breast disease (benign controls) and 397 women with a negative screening mammogram (well controls) enrolled in the Mammograms and Masses Study. Associations between the inflammatory markers and mammographic density were evaluated separately for benign and well controls through correlation analyses and linear regressions. Age-adjusted mean CRP levels were higher among benign controls (2.07 mu g/ml) compared to well controls (1.63 mu g/ml; P = 0.02), while IL-6 and TNF-alpha levels were similar between groups. Using linear regression, IL-6, TNF-alpha, and CRP were not statistically significantly associated with dense breast area within either group. Statistically significant positive associations were observed between all three markers and nondense breast area in both groups; statistically significant negative associations were observed between IL-6 and percent density among benign controls, and between all three markers and percent density among well controls. These associations were all attenuated and non-significant upon adjustment for body mass index. IL-6, TNF-alpha, and CRP levels were not independently associated with dense breast area, nondense breast area, or percent density in this study population. Our results suggest that these inflammatory factors do not impact breast carcinogenesis through independent effects on mammographic density.