Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 13, Pages 6688-6695Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b00847
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Funding
- 973 program [2013CB933901]
- NSF China [21272196, 21305116, 91429301, 31420103910, 31330047, 31221065]
- Natural Science Foundation of Fujian Province of China [2011J06004]
- PCSIRT
- State Key Laboratory of Chemo/biosensing and Chemo-metrics [2012002]
- National Scientific and Technological Major Project [2013ZX10002-002]
- Hi-Tech Research and Development Program of China (863program) [2012AA02A201]
- 111 Project [B12001]
- Science and Technology Foundation of Xiamen [3502Z20130027]
- National Science Foundation of China for Fostering Talents in Basic Research [J1310027]
- State Key Laboratory of Cellular Stress Biology, Xiamen University
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Inflammation causes significant morbidity and mortality, necessitating effective in vivo imaging of inflammation. Prior approaches often rely on combination of optical agents with entities specific for proteinaceous biomarkers overexpressed in inflammatory tissues. We herein report a fundamentally new approach to image inflammation by targeting lysosomes undergoing acidification in inflammatory cells with a sialic acid (Sia) conjugated near-infrared profluorophote (pNIR). Sia pNIR contains a sialic acid domain for in vivo targeting of inflamed tissues and a pNIR domain which isomerizes into fluorescent and optoacoustic species in acidic lysosomes. Sia pNIR displays high inflammation-to-healthy tissue signal contrasts in mice treated with Escherichia coli, Staphylococcus aureus, or lipopolysaccharide. In addition, inflammation-associated fluorescence is switched off upon antibiotics treatment in mice. This report shows the potentials of Sia pNIR for activatable dual-modality inflammation imaging, and particularly the use of lysosomes of inflamed cells as a previously unappreciated biomarker for inflammation imaging.
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