Journal
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
Volume 49, Issue 1, Pages 117-126Publisher
UNIV SAO PAULO, CONJUNTO QUIMICAS
DOI: 10.1590/S1984-82502013000100013
Keywords
Fluorouracil/determination; Nanoparticles; High performance liquid chromatography/quantitative analysis/method validation
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Funding
- CAPES
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The objective of this work was to develop and validate a rapid high performance liquid chromatography (HPLC) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10: 90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated using a photodiode array detector at a wavelength of 265 nm. The method was shown to be specific and linear in the range of 0.1-10 mu g/mL (r = 0.9997). The precision (intra-and inter-day) was demonstrated because the maximum relative standard deviation was 3.51%. The method is robust relative to changes in flow rate, column and temperature. The limits of detection and quantitation were 10.86 and 32.78 ng/mL, respectively. The method fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of 5-FU in polymeric nanoparticles.
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