Journal
BRAZILIAN JOURNAL OF MICROBIOLOGY
Volume 44, Issue 2, Pages 569-576Publisher
SPRINGER
DOI: 10.1590/S1517-83822013000200037
Keywords
Aspergillus awamori 2B.361 U2/1; cellulases; hemicellulases; beta-glucosidase; nitrogen nutrition
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Funding
- FINEP
- Brazilian Research Council (CNPq)
- Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES)
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The production of xylanase, beta-xylosidase, ferulic acid esterase and beta-glucosidase by Aspergillus awamori 2B.361 U2/1, a hyper producer of glucoamylase and pectinase, was evaluated using selected conditions regarding nitrogen nutrition. Submerged cultivations were carried out at 30 degrees C and 200 rpm in growth media containing 30 g wheat bran/L as main carbon source and either yeast extract, ammonium sulfate, sodium nitrate or urea, as nitrogen sources; in all cases it was used a fixed molar carbon to molar nitrogen concentration of 10.3. The use of poor nitrogen sources favored the accumulation of xylanase, beta-xylosidase and ferulic acid esterase to a peak concentrations of 44,880; 640 and 118 U/L, respectively, for sodium nitrate and of 34,580, 685 and 170 U/L, respectively, for urea. However, the highest beta-glucosidase accumulation of 10,470 U/L was observed when the rich organic nitrogen source yeast extract was used. The maxima accumulation of filter paper activity, xylanase, beta-xylosidase, ferulic acid esterase and beta-glucosidase by A. awamori 2B. 361 U2/1 was compared to that produced by Trichoderma reesei Rut-C30. The level of beta-glucosidase was over 17-fold higher for the Aspergillus strain, whereas the levels of xylanase and beta-xylosidase were over 2-fold higher. This strain also produced ferulic acid esterase (170 U/L), which was not detected in the T. reesei culture.
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