Journal
BRAZILIAN JOURNAL OF MICROBIOLOGY
Volume 43, Issue 2, Pages 834-843Publisher
SPRINGER
DOI: 10.1590/S1517-83822012000200050
Keywords
Listeria monocytogenes; prfA; Virulence; Microarray; Biofilms; Co-culture; Gene Expression
Categories
Funding
- Genotypic Technologies, Bangalore, India
Ask authors/readers for more resources
Majority of studies concerning the gene expression of Listeria monocytogenes have been done on pure culture states. Our objective was to study L. monocytogenes in a co-cultured state and to understand if microbes in their natural state of existence are different in their expression than that of the purely cultured lab grown forms. For a long period discussions have been on the expression of prfA, (which is a virulence gene regulator) in a mammalian host and its role in causing the switch from a saprophytic to pathogenic form of L. monocytogenes. We, in this paper for the first time report the expression of prfA and other virulence genes by L. monocytogenes under different extracellular conditions, and also as a pure culture biofilms, that is different from the previous reports. We also report that the expression of prfA seems to vary considerably when co-cultured with Bacillus subtilis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available