Journal
BRAZILIAN JOURNAL OF INFECTIOUS DISEASES
Volume 18, Issue 6, Pages 672-674Publisher
CONTEXTO
DOI: 10.1016/j.bjid.2014.08.004
Keywords
Multiplex-PCR; Clostridium difficile; HRM; Toxin gene profiling
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Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C. difficile polymerase chain reaction ribotypes 027/078 strains, we used high resolution melting and multiplex polymerase chain reaction. Among 116 screened patients, 11 were positive for C. difficile. The polymerase chain reaction ribotypes 027/078 strains were not identified in this study. (C) 2014 Elsevier Editora Ltda. All rights reserved.
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