4.1 Article

Evaluation of Red Cabbage Anthocyanins after Partial Purification on Clay

Journal

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
Volume 54, Issue 6, Pages 1349-1356

Publisher

INST TECNOLOGIA PARANA
DOI: 10.1590/S1516-89132011000600022

Keywords

Thin-layer chromatography; spectrophotometry; purification; batch conditions

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The aim of this work was to evaluate the red cabbage anthocyanins quality after purification by static adsorption assays on clay (Tonsil Terrana 580FF). Thin-layer chromatography analysis and scan spectrometry on UV-Visible showed a good dye stability of the pigment interacting with the clay. The use of pH 3.0 buffer during the batch assays provided a protective effect on the cabbage anthocyanins, limiting the dye acid hydrolyses. Analyses of the reducing sugars contents of the extract showed that clay retained 20% average of total free sugars under the test conditions.

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