Journal
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
Volume 54, Issue 4, Pages 691-700Publisher
INST TECNOLOGIA PARANA
DOI: 10.1590/S1516-89132011000400007
Keywords
marine algae; sulfated macromolecules; chemical analysis; APTT test
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Funding
- National Scientific and Technological Development Council (CNPq)
- Northeast Biotechnology Network (RENORBIO)
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- Ceara State Scientific and Technological Development Foundation (FUNCAP)
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The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg(-1)). The yield was similar to 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl, respectively. Only fraction F II was active (24.62 IU mg(-1)), with high sulfate content (23.79%) and number of molecular mass peaks. Therefore, the APTT of a fraction isolated from C. cupressoides was less potent than HEP.
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