Journal
BRAIN STRUCTURE & FUNCTION
Volume 219, Issue 2, Pages 631-640Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00429-013-0523-9
Keywords
Stereology; Juxtacellular; Electron microscopy; Single cell; Neuronal digital reconstruction
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Funding
- Medical Research Council (UK), Parkinson's UK [G-0601]
- Comision Nacional de Investigacion Cientifica y Tecnologica (Fondecyt, Conicyt, Chile) [11100433]
- Medical Research Council [MC_U138197109, MC_U138164490] Funding Source: researchfish
- Parkinson's UK [J-0901, G-0601] Funding Source: researchfish
- MRC [MC_U138164490, MC_U138197109] Funding Source: UKRI
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Determining the number and placement of synaptic inputs along the distinct plasma membrane domains of neurons is essential for explaining the basis of neuronal activity and function. We detail a strategy that combines juxtacellular labeling, neuronal reconstructions and stereological sampling of inputs at the ultrastructural level to define key elements of the afferent 'synaptome' of a given neuron. This approach provides unbiased estimates of the total number and somato-dendritic distribution of synapses made with individual neurons. These organizational properties can be related to the activity of the same neurons previously recorded in vivo, for direct structure-function correlations at the single-cell level. The approach also provides the quantitative data required to develop biologically realistic models that simulate and predict neuronal activity and function.
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