Journal
BRAIN RESEARCH BULLETIN
Volume 94, Issue -, Pages 56-62Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.brainresbull.2013.02.007
Keywords
Cerebral ischemia; miRNA; MMP9; ERIC
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Funding
- Program for the Science and Technology of Nanjing [200901081]
- National Natural Science Foundation of China [81170714]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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Matrix metallinoprotease-9 (MMP9) plays a key role in the pathogenesis of post-ischemic blood brain barrier (BBB) disruption and the formation of lesions after cerebral ischemia. In this study we investigate the effect of brain-specific miRNAs on MMP-9 protein level in the rat hippocampus following cerebral ischemia and its underlying mechanism. Cerebral ischemia significantly upregulated miR-21 and -224 in the hippocampus; however, expression of miR-122 and -338-3p was not significantly affected by ischemia. Silencing of miR-21, but not -224, reduced MMP9 protein level after cerebral ischemia. Downregulation of extracellular signal-regulated kinase (ERK) signaling using the ERK inhibitor U0126 and the calcium-channel blocker ketamine inhibited the upregulation of miR-21 expression and MMP9 protein level after cerebral ischemia. The study suggests that cerebral ischemia up-regulates expression level of miR-21, which is involved in ERIC-stimulated upregulation of MMP9 following cerebral ischemia via a calcium-dependent mechanism. (C) 2013 Elsevier Inc. All rights reserved.
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