Journal
BRAIN RESEARCH BULLETIN
Volume 82, Issue 5-6, Pages 308-312Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.brainresbull.2010.06.006
Keywords
Glioma stem cell; Transdifferentiation; Vascular endothelial cell
Categories
Funding
- National Basic Research Program of China [2010CB529403]
- National Natural Science Foundation of China [30973081, 30872654]
- Natural Science Foundation of Jiangsu Province, China [BK2007507]
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The transdifferentiation of normal stem cells in many kinds of tissues or organs has been studied. However, whether glioma stem cells can transdifferentiate is seldom reported. Meanwhile, the mechanism of angiogenesis in tumors is in disputations, and it is still unknown that whether glioma stem/progenitor cells (GSPCs) participate into angiogenesis in glioma. In this study, we cultivated GSPCs in endothelial differentiation medium for 10 days and found they present to be the typical flagstone appearance of vascular endothelial cell (VEC); when cultured on Matrigel, GSPCs gradually formed tubular-like structures in vitro, and cells, which formed the tubular-like structures, showed similar ultrastructural characteristics of VEC under a transmission electron microscope. Furthermore, when cultured in hypoxia or oxygen-glucose deprivation for 4 h, GSPCs transcribed and expressed molecular markers of VEC, including CD31, CD34 and vWF. These results indicated that GSPCs could participate into angiogenesis of glioma by transdifferentiating into VEC-like cells. (C) 2010 Elsevier Inc. All rights reserved.
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