Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 10, Pages 5167-5172Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b00890
Keywords
-
Categories
Funding
- Spanish MINECO [MAT2011-25870]
- Spanish MINECO through the Severo Ochoa Centers of Excellence Program [SEV-2013-0295]
- Universitat Autonoma de Barcelona
- ICREA Funding Source: Custom
Ask authors/readers for more resources
In this article, a novel aptasensor for ochratoxin A (OTA) detection based on a screen-printed carbon electrode (SPCE) modified with polythionine (PTH) and iridium oxide nanoparticles (IrO2 NPs) is presented. The electrotransducer surface is modified with an electropolymerized film of PTH followed by the assembly of IrO2 NPs on which the aminated aptamer selective to OTA is exchanged with the citrate ions surrounding IrO2 NPs via electrostatic interactions with the same surface. Electrochemical impedance spectroscopy (EIS) in the presence of the [Fe(CN)(6)](-3/-4) redox probe is employed to characterize each step in the aptasensor assay and also for label-free detection of OTA in a range between 0.01 and 100 nM, obtaining one of the lowest limits of detection reported so far for label-free impedimetric detection of OTA (14 pM; 5.65 ng/kg). The reported system also exhibits a high reproducibility, a good performance with a white wine sample, and an excellent specificity against another toxin present in such sample.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available