cPLA2α knockout mice exhibit abnormalities in the architecture and synapses of cortical neurons

Cytosolic phospholipase A2 alpha (cPLA2 alpha) affects membrane fluidity and permeability by catalyzing the hydrolysis of membrane phospholipids. We hypothesize that cPLA2 alpha deficiency induces rigidity and architectural changes in cell membranes, especially in large cortical neurons. These membrane changes are discernible using light and electron microscopy. Through careful comparison with wild-type counterparts, we observed significant morphological changes in cortical neurons of cPLA2 alpha knockout mice. These changes included the following: (1) increased numbers of nucleoli and enlarged nuclei, (2) narrower spaces between the inner and outer nuclear membranes, (3) reduced numbers of nuclear pores and altered nuclear pore structure, and (4) morphological changes in synaptic clefts. These results further suggest that cPLA2 alpha and its cleaved arachidonic acids play important roles in cortical neuronal maturation and in normal neurochemical processes. Published by Elsevier B.V.