Journal
BRAIN RESEARCH
Volume 1464, Issue -, Pages 61-72Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2012.04.039
Keywords
Parkinson's disease; Neurogenesis; Neural stem cell; Neural progenitor cell; Cell culture
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Funding
- NIH/NINDS grant [NS055165]
- Maren Regenerative Medicine Fund
- Thompson Regenerative Medicine Fund
- McKinney Regenerative Medicine Funds
- National Parkinson Foundation
- NIH/NINDS
- NPF Center of Excellence at the University of Florida
- Citizens United for Research in Epilepsy grant
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The success of cellular therapies for Parkinson's disease (PD) will depend not only on a conducive growth environment in vivo, but also on the ex vivo amplification and targeted neural differentiation of stem/progenitor cells. Here, we demonstrate the in vitro proliferative and differentiation potential of stem/progenitor cells, adult human neural progenitor cells (AHNPs) isolated from idiopathic PD postmortem tissue samples and, to a lesser extent, discarded deep brain stimulation electrodes. We demonstrate that these AHNPs can be isolated from numerous structures (e.g. substantia nigra, SN) and are able to differentiate into both glia and neurons, but only under particular growth conditions including co-culturing with embryonic stem cell-derived neural precursors (ESNPs); this suggests that PD multipotent neural stem/progenitor cells do reside within the SN and other areas, but by themselves appear to lack key factors required for neuronal differentiation. AHNPs engraft following ex vivo expansion and transplantation into the rodent brain, demonstrating their regenerative potential. Our data demonstrate the presence and capacity of endogenous stem/progenitor cells in the PD brain. (c) 2012 Elsevier B.V. All rights reserved.
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