Journal
BRAIN RESEARCH
Volume 1422, Issue -, Pages 39-45Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2011.08.076
Keywords
Signal transducers and activators of transcription 3 (STAT3); Ischemic preconditioning; Transient global ischemia; Hippocampus
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Funding
- Ministry of Education, Science and Culture of Japan [19390375]
- Grants-in-Aid for Scientific Research [19390375] Funding Source: KAKEN
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The signal transducers and activators of transcription 3 (STAT3) has been suggested to have neuroprotective roles. However, its role in ischemic preconditioning (PC) is still obscure. In this study, we examined the phosphorylation status of ser727-STAT3, which is necessary for activation of STAT3, and its roles in a rat global ischemia model with or without PC. PC was induced by 3 min of nonlethal ischemia 48 h before 5 min of lethal ischemia. Western blot analysis showed that phospho-ser727-STAT3 significantly increased from 8 to 48 h after nonlethal ischemia, while it increased only for 1 h after lethal ischemia and returned to the baseline within 24 h. In the preconditioned brains, phospho-ser727-STAT3 was induced at 1 to 4 h after lethal ischemia, and decrease of its levels delayed compared to the nonconditioned brains. Immunohistochemistry revealed that phospho-ser727-STAT3 was expressed mainly in CA1 neurons after nonlethal ischemia. Additionally, STAT3 inhibitor peptide treatment prevented PC induced-neuroprotection. These results indicate that phosphorylation of ser727-STAT3 plays an important role in PC induced- neuroptotection. (C) 2011 Elsevier B.V. All rights reserved.
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