Journal
BRAIN RESEARCH
Volume 1344, Issue -, Pages 13-24Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2010.04.065
Keywords
Binding protein; Cell adhesion; Development; Localization; Protocadherin; Retina
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Funding
- Ministry of Education, Culture, and Technology [18659055]
- Ministry of Health, Labour, and Welfare [H21-G-209]
- Kwansei Gakuin University
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Protocadherin-19 has been implicated in some neurological diseases, but even the basic properties of this protocadherin have not yet been characterized well. Hence, various basic properties of chicken protocadherin-19 were examined to elucidate its biological role. The protocadherin-19 expressed in L cells was localized at the intercellular contact sites and showed Ca2+-dependent homophilic cell aggregation activity that was relatively weak but showed stringent specificity. The results of a pull-down assay using fusion proteins of the cytoplasmic domain and glutathione S-transferase yielded specifically bound proteins. In the bound fractions, liquid chromatography-mass spectrometry identified Nck-associated protein 1 and cytoplasmic FMP1 interacting protein 2, which have been reported to bind to glutathione S-transferase fused with the cytoplasmic domain of OL-protocadherin, suggesting that these proteins generally have affinity for 8 protocadherins. Protocadherin-19 was mainly expressed in the central nervous system. In the chicken retina, protocadherin-19 was expressed as early as embryonic day 5 and was localized in the ganglion cell layer, inner plexiform layer, and optic nerve layer. Chicken protocadherin-19 was co-localized with syntaxin 1 in inner plexiform layer and was also expressed in the optic nerve and in specific layers of optic tectum. These results suggest that protocadherin-19 plays a role as an adhesion protein in optic nerve fiber bundling, optic nerve targeting, and/or synapse formation. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.
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