Journal
BRAIN RESEARCH
Volume 1301, Issue -, Pages 152-162Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2009.09.018
Keywords
ALS; Mutant SOD1; Rat; Blood-brain barrier; Blood-spinal cord barrier; Evans blue
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Funding
- FNRS [3.4.545.05 F]
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Blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) impairment is an additional accident occurring during the amyotrophic lateral sclerosis (ALS) progression. In this work, we aimed to decipher if BBB/BSCB leakage appeared before critical detrimental events and could serve as a marker preceding clinical symptoms. Three different BBB leakage markers: Evans blue, IgG and hemosiderin, were used to look at the SOD1-linked ALS rat model at presymptomatic and symptomatic stages. Although IgG and hemosiderin could be detected at presymptomatic stage, Evans blue extravasation which fits best with BBB/BSCB impairment could only be seen at symptomatic stages. BBB/BSCB impairment was further substantiate by showing at symptomatic stages decreased mRNA expression of ZO-1 and occludin as well as agrin, a basal membrane constituent. Electron microscopic data substantiate a toxic environment around endothelial cell and peri-vascular swollen astrocyte end-feet showing oedema-linked BBB opening. (C) 2009 Elsevier B.V. All rights reserved.
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