Journal
BRAIN RESEARCH
Volume 1234, Issue -, Pages 198-205Publisher
ELSEVIER
DOI: 10.1016/j.brainres.2008.07.121
Keywords
BBB; VEGF; Evans blue dye; Tetanus toxin fragment C; AAV2/5; SOD1(G93A) mice
Categories
Funding
- NIA
- NINDS
- ALS Association
- Angel Fund
- Al-Athel ALS Research Foundation
- ALS Research Foundation
- Muscular Dystrophy Association
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Entry of most compounds into the CNS is impeded by the blood-brain barrier (BBB). Because vascular endothelial growth factor (VEGF) is important in the formation and maintenance of the BBB and is known to modulate BBB permeability in newborn rodents, we tested the hypothesis that VEGF may enhance BBB permeability in adult mice. We examined the effect of VEGF on the CNS distribution of three different agents: a small molecule (Evans blue dye) that is known to bind plasma proteins, an exogenous protein (tetanus toxin fragment C; TTC), and a viral vector (recombinant adeno-associated virus serotype 2/5 marked with lacZ; rAAV2/S-lacZ). Pretreatment with VEGF (20 mu g; i.v.) increased permeability of the BBB to Evans blue dye and TTC as detected by augmented concentrations of these substances in the cerebrum, brainstem, and spinal cord. By contrast, VEGF did not alter BBB permeability to AAV2/S-lacZ, as defined by beta-galactosidase activity assay. These data demonstrate the potential utility of VEGF for pharmacological modulation of the BBB, and indicate that increase in BBB permeability mediated by VEGF is limited by the size of the delivered substance. (C) 2008 Elsevier B.V. All rights reserved.
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