Journal
PLANT SIGNALING & BEHAVIOR
Volume 5, Issue 11, Pages 1517-1519Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/psb.5.11.13711
Keywords
Chloroplast signalling; RNA import; viroids; RNA localization; nucleus; non-coding RNAs
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The import of diverse nucleus-encoded proteins into chloroplasts is crucial for plant life. Although this crosstalk is mainly dependent on specific transit peptides, it has been recently reported that a non protein-coding RNA (ncRNA) based on a viroid-derived sequence (vdRNA) and acting as a 5' UTR-end mediates the functional import of GFP-mRNA into chloroplasts. This observation unearths a novel plant cell-signaling pathway able to control the accumulation of the nuclear-encoded proteins in this organelle. The mechanisms regulating this chloroplast-specific localization remain yet unclear. To unravel the functional nature of this chloroplastic signal, here we dissect the 5' UTR-end responsible for the chloroplast targeting. A confocal microcopy analysis in Nicotiana benthamiana leaves of the transcripts expression carrying partial deletions of the 5' UTR-end indicates that an internal 110 nucleotides-length fragment is sufficient to mediate the traffic of functional GFP-mRNA into chloroplasts. However, the capability of this motif to act as a chloroplastic localization signal was enhanced when fused to either the 5' or the 3' region of the vd-5' UTR sequence. These findings suggest that the chloroplast-specific RNA targeting is dependent on a structural motif rather than on the RNA sequence.
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