Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 13, Pages 6475-6478Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b01657
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Funding
- Collaborative Innovation Center of Suzhou Nano Science and Technology
- Major Program of Development Foundation of Hefei Center for Physical Science and Technology
- Hefei Normal University [2015QN08]
- National Natural Science Foundation of China [21175122, 21375121]
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Alkaline phosphatase (ALP)-catalyzed hydrogelation has been extensively explored and found wide applications. Spectroscopic and electrochemical approaches are commonly employed for the detection of ALP activity. Herein, by rational design of a fluorescence probe Fmoc-K(FITC)FFYp (P1) (where FITC is fluorescein), we incorporated sol gel transition with fluorescence turn-off and developed a new method for quantitative sensing ALP activity in vitro and in living cells. Under the catalysis of ALP, P1 was converted to hydrogelator Fmoc-K(FITC)FFY (1) which self-assembles into nanofibers to form Gel I. Accompanying this sol gel transition, the fluorescence emission of P1 was turned off. Our assay was employed to detect ALP activity over the range of 0-2.8 U/mL with a limit of detection (LOD) of 0.06 U/mL. ALP-inhibitor-treated cell imaging indicated that P1 could be applied for sensing ALP activity in living cells. Our method provides a new option for real time and quantitative sensing ALP activity in vitro and even in living cells.
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