Journal
BRAIN BEHAVIOR AND IMMUNITY
Volume 34, Issue -, Pages 86-97Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbi.2013.07.174
Keywords
CD200; Blood brain barrier permeability; Inflammation; Microglial activation; Lipopolysaccharide; Claudin
Categories
Funding
- Science Foundation Ireland
- Health Research Board (Ireland)
- Dublin Region Higher Education Alliance
- Strategic Innovation Fund from the Higher Education Authority
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The interaction between CD200, expressed on several cell types, and its receptor CD200R, expressed on cells of the myeloid lineage, has been shown to be an important factor in modulating inflammation in macrophage function in several conditions including colitis and arthritis. More recently its modulatory effect on microglial activation has been identified and CD200-deficiency has been associated with increased microglial activation accompanied by increased production of inflammatory cytokines. The response of glia prepared from CD200-deficient mice to stimuli like lipopolysaccharide (LPS) is markedly greater than the response of cells prepared from wildtype mice and, consistent with this, is the recent observation that expression of Toll-like receptor (TLR)4 and signalling through NP kappa B are increased in microglia prepared from CD200-deficient mice. Here we show that glia from CD200-deficient mice are also more responsive to interferon-gamma (IFN gamma) which triggers classical activation of microglia. We investigated the effects of CD200-deficiency in vivo and report that there is an increase in expression of several markers of microglial activation including tumor necrosis factor (TNE)-alpha, which is a hallmark of classically-activated microglia. These changes are accompanied by increased IFN gamma, and the evidence suggests that this is produced by infiltrating cells including T cells and macrophages. We propose that these cells enter the brain as a consequence of increased blood brain barrier (BBB) permeability in CD200-deficient mice and that infiltration is assisted by increased expression of the chemokines, monocyte chemotactic protein-1 (MCP-1), IFN gamma-induced protein-10 (IP-10) and RANTES. This may have implications in neurodegenerative diseases where BBB permeability is compromised. (C) 2013 Elsevier Inc. All rights reserved.
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